The effect of interleukin-17 on podocyte-associated factors and apoptosis and molecular mechanism

Abstract

Objective To investigate the effect of interleukin(IL)-17 on podocyte-associated factors and apoptosis in podocytes and explore the molecular mechanism. Methods The podocytes were used as the object of study.The apoptosis of podocytes induced by IL-17 in a dose(1 nm/ml, 10 nm/ml, 50 nm/ml, 100 nm/ml) and time(12 h, 24 h, 48 h, 72 h) way and the protein expression of Fas and FasL of podocytes induced by IL-17 were detected by flow cytometrey.The podocytes were divided into the blank control group and 100 ng/ml IL-17 induction group.The expressions of mRNA of Nephrin, WT1, Synaptopodin, Podocylaxin, Fas and FasL were measured by Real-time RT PCR.The proteins of WT1, Caspases8, Caspases3 in podocyte were detected by immunocytochemistry method. Results IL-17 promoted the apopotosis of podocyte in a dose and time way(P 0.05). Conclusion IL-17 may cause renal injure by inducing the apoptosis of podocyte and decreasing the expression of Podocylaxin in podocyte. Key words: Interleukin-17; Podocyte; Apoptosis; Fas; Podocylaxin,