The Effect of Insulin-Like Growth Factor-I of Pregnant Crossbred Mare Serum and Insulin-Like Growth Factor-I Recombinant Mouse on Estrous Cycles and Litter Sizes of Mice (Mus musculus)

Tjuk Imam Restiadi,Imam Mustofa,Sri Mulyati,Suzanita Utama

doi:10.18502/kls.v3i6.1126

Abstract

The research was conducted to determine the effect of insulin-like growth factor-I (IGF-I) derived from pregnant crossbred mare serum (PMS) on estrous cycle and litter size in mice (Mus musculus). The research was arranged by Completely Randomized Design (CRD) with seven treatments and five replications. The treatments were control (CO)= physiological NaCl, treatment (T) 1 = 10 ng/ml of IGF-I pregnant mares crossbred serum (PMS), T2 = 20 ng/ml of IGF-I PMS, T3 = 40 ng/ml of IGF-I PMS, T4 = 10 ng/ml of IGF-I recombinant mouse, T5 = 20 ng/ml of IGF-I recombinant mouse, and T6 = 40 ng/ml of IGF-I recombinant mouse. Serie I the subject of this research were 35 female mice. Observed variables included estrous cycle phases that proestrus, estrus, metestrus, and diestrus phase. Serie II for the objects of this research were 35 female mice and 21 male mice in the same treatment on litter size in mice. The data were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS did not significantly affect (p>0.05) on proestrus, metestrus, and diestrus phase. The addition of IGF-I PMS 40 ng/ml was extended (p<0.05) the estrus phase. It showed that there was not significantly difference (p>0.05) between the effect of IGF-I PMS and IGF-I recombinant mouse with the mice estrous cycles. The result of IGF-I derived from PMS and IGF-I recombinant mouse on litter size in mice did not significantly difference (p>0.05). Test results shows significant differences between control and 40 ng/ml of IGF-I PMS. Keywords: IGF-I, IGF-I pregnant crossbred mare serum, estrous cycle, litter sizes, Mus musculus

Highlights

Insulin-like growth factor-I (IGF-I) is one of bioactive material, a supplementation of insulin-like growth factor-I (IGF-I) in maturation and culture medium can stimulate and increase the number of oocyte
Insulin-like growth factor-I (IGF-I) is one of bioactive material, a supplementation of IGF-I in maturation and culture medium can stimulate and increase the number of oocyte. It can increase the result of in vitro fertilization (IVF) and the amount of some livestock’s embryo, such as pigs [1], cows [2], and buffaloes [3], which run into blastocyst
The objects for the effect of IGF-I on the amount of mice litter acquisition were 35 female mice aged 30-35 days and 21 male mice aged 2 months, and they were adapted for seven days

Summary

Introduction

Insulin-like growth factor-I (IGF-I) is one of bioactive material, a supplementation of IGF-I in maturation and culture medium can stimulate and increase the number of oocyte. It can increase the result of in vitro fertilization (IVF) and the amount of some livestock’s embryo, such as pigs [1], cows [2], and buffaloes [3], which run into blastocyst. One of the most important aspects in animal reproduction is estrus cycle Monitoring this cycle plays an important role for fertilization and reproduction as it increases the number of animal population [5]

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Results

Conclusion