Abstract
Selenium is a microelement of big importance for male reproduction. As a part of the antioxidative enzyme gluthationperoxidase and structural Se proteins, it plays pivotal role in the defense of spermatozoa against generated ROS and in ensuring of its motility. During the last years the interests to the organic forms of selenium was enhanced because of its better biological utilization. The present work aimed to study the effect of injective application of organic compound selenopyran in rams on the distribution of selenium content in blood and sperm and on the changes in sperm quality. The experiment was conducted with 5 rams from the Synthetic Population Bulgarian Milk breed at the age between 3-7 years and live weight 85-90 kg. The animals were injected once with an oil solution of the selenopyran in dose of 0.1mg/kg live weight (selenium content 24%) 45 days before starting the breeding season. The blood was collected before treatment and 45 days thereafter. At the same time the first and second ejaculates of rams were collected using artificial vagina and analyzed by Sperm Class Analyzer. The selenium content was measured in plasma, blood cells and sperm by atomic absorption spectrometry using SpectrAA 55B double beam spectrometer (Varian, Inc.). The results showed that one injection of organic compound of selenium - selenopyran in dose of 0.1mg/kg live weight ensured the increase and support of high level of selenium in blood (plasma and blood cells) and in sperm during investigated period. That lead to the proper spermatogenesis in testis and allowed the production of qualitative ejaculates with higher number of total spermatozoa as well as of spermatozoa with progressive motility.
Highlights
During the last decades there are new evidences of selenium importance for the male reproduction, especially for the properly spermatogenesis
Salt and mineral licks were placed in pens (EuroLick MultiVit®), as the concentration of Se in licks was 10 mg per kg. 45 days before breeding season the experimental rams were injected once subcutaneously with oil solution of selenopyran (9-phenylymmetrical octahydroselenoxanthene) in dose of 0.1 mg /kg live weight
As mentioned in previous investigations (Abadjieva et al, 2014), the advantages of selenopyran are the lower toxicity in comparison with sodium selenite (LD50=1600 mg/kg against LD50=3.25 mg/kg) and ability to slowly liberate the selenium according to the needs of the organisms (Boryaev and Kravchenko, 2006)
Summary
During the last decades there are new evidences of selenium importance for the male reproduction, especially for the properly spermatogenesis. More than 25 selenoproteins were identified in the live organisms. Most part of them occurs in the male reproductive system at tissue (testis, epididymal epithelium), cellular (intracellular membranes) and subcellular level (sperm nucleus, mitochondrial capsule) (Ahsan et al, 2014). Se concentrations in rodent testes exceed that of other organs, except kidneys (Schriever et al, 2009). According to Kehr et al (2009) the distribution of Se in midpiece and head of spermatozoa is 4:1. Spermatozoa may be more vulnerable to oxidative stress if the Se content in selenoproteins is low and likely decreases the possibility of fertilization (Beckett and Arthur, 2005)
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