The effect of injecting HEPES buffer into eggs of broiler breeder ROSS 308 before storage on the hatching characteristics and chick quality

Abstract

The experiment studies the injection of a HEPES buffer into the stored eggs in three groups (14 days for the first group, 7 for the second, and 4 for the third). It also investigates the prolongation of the storage period of hatching eggs by injecting them with HEPES buffer in addition to its effect on the qualitative hatching characteristics and chicks specification. The groups of eggs were injected with HEPES buffer of (H50 mM, H75 mM) concentration and then stored for 14, 7 and 4 days. The eggs were brought in groups from the fields of broiler mothers, ROSS 308 breed, from the Modern Poultry Company in (Wasit, Azizia). The first group of 294 days old (42 weeks) was stored for 14 days. The second group of age 308 days (44 weeks) was stored for 7 days and the third, at the age of 311days, was stored for 4 days. The eggs were divided into 15 treatments of 30 eggs each. At the end of the storage period, the results of the experiment show that injecting HEPES buffer with a concentration of H50 and H75 helps reduce the pH value of the white and yolk in the eggs of the treatments that are stored for 4 and 7 days. As for the treatments of eggs stored for 14 days, the buffer does not help to reduce the pH value of the white and yolk at the end of the storage period. The results of the experiment also show significant superiority of (P≤0.05) in the percentage of hatching of the fertilized eggs injected with the buffer. The results also show there is an effect of the injection on the chick weight (gm) and chick length CRL (cm) in the treatments of the eggs stored for 4, 7 and 14 days. The results of the internal specifications of the hatched chicks show superiority in the relative weight of the gizzard, the glandular stomach, and the intestines of the injected eggs (14 and 4 days storage). The same characteristics were superior with the injection of H75 buffer into treatments of 7 days stored eggs.