Abstract
Objective To investigate the effect of knocking down the expression of replication protein A1 (RPA1) on radiosensitivity of human esophageal carcinoma cells.Methods Antisense oligonuleotides of RPA1,mediated by LipofectamineTM 2000,were transfected into TE-1 cells.Four groups were set up:blank control group,mock group ( only LipofectamineTM 2000),negative control group and antisense transfected group at 24,48,72,96 h.Tge expression levels of RPA1 mRNA in TE-1 cells were determined by using reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time PCR (qRT-PCR),and those of RPA1 protain by using Western blotting.TE-1 cells were irradiated with 6 MV X-ray at the dose of 6 Gy.Cell counting Kit-8 (CCK-8) method was used to analyze the radiosensitivity of TE-1 cells after tansfected with antisense oligonuleotides of RPA1.Results RPA1 mRNA and protein were down-regulated in antisense transfected group as compared with other groups.CCK-8 method revealed that antisense oligonuleotides of RPA1 could improve the radiosensitivity of TE-1 cells as compared with other groups (P ＜ 0.05).Conclusion Antisense oligonuleotides of RPA1 can induce the down-regulation of RPA1 in esophageal carcinoma cells,and improve the radiosensitivity of esophageal carcinoma cells. Key words: Esophageal carcinoma; Replication protein A1 ; Radiosensitivity
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