Abstract
BackgroundEntamoeba histolytica (E. histolytica) causes amoebiasis, which is a disease with significant morbidity and mortality. Phagocytic cells and cytokines appear to be important in amoebiasis, but very little is known about the influence of these cells and cytokines in protozoan infections. The aim of this study was to analyse the supernatant of cultures of mononuclear (MN) cells with E. histolytica to determine: 1) the levels of the cytokines IFN-γ and TGF-β, and 2) the amoebicidal activity of MN cells after incubation with cytokines.MethodsBlood samples were collected from 30 volunteer donors. The cytokine concentrations in MN cells culture supernatants, superoxide release, leukophagocytosis, amoebicide activity, intracellular calcium release and apoptosis were analysed.ResultsThe IFN-γ concentrations were 6.22 ± 0.36 and TGF-β concentrations were 17.01 ± 2.21 in cells–trophozoite culture supernatants. MN cells, independently of cytokines, in the presence of amoeba increase the superoxide release. In the absence of cytokines, the ingestion of MN cells by amoebae was higher. In the presence of IFN- γ or TGF- β, a lower ingestion of MN cells was observed by amoebae. MN cells treated with cytokines exhibited higher amoebicide and apoptosis indexes. The incubation of cytokines increased the intracellular calcium release by MN cells.ConclusionsThese results suggest that cytokines play a beneficial role for the host by activating MN cells against E. histolytica. The increased death of amoebae during the leukophagocytosis suggests that both cytokines (IFN-γ and TGF-β) can modulate the functional activity of MN cells and that these cytokines probably are important in the control of amoebic infections.
Highlights
Entamoeba histolytica (E. histolytica) causes amoebiasis, which is a disease with significant morbidity and mortality
Proinflammatory cytokines are responsible for the recruitment of neutrophils [4] and the release of inflammatory mediators that promote the migration of neutrophils and macrophages [5, 6]
The IFN-γ and TGF-β did not alter the viability of MN cells
Summary
Entamoeba histolytica (E. histolytica) causes amoebiasis, which is a disease with significant morbidity and mortality. Phagocytic cells and cytokines appear to be important in amoebiasis, but very little is known about the influence of these cells and cytokines in protozoan infections. Acute amoebiasis lesions are characterized by the presence of inflammatory cells that are recruited by proinflammatory signals produced by epithelial cells and other host cells. Moraes et al Parasites & Vectors (2015) 8:413 for the destruction of microorganisms beginning with the adhesion of these immune cells to the cell membrane. This interaction can be enhanced by hormonal and immunological factors [7,8,9,10,11,12]
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