The effect of hydrogen peroxide and subsequent resveratrol application to CAD-CAM blocks on the cell viability of fibroblasts.

Abstract

The aim is to assess viability of fibroblasts exposed to 2 CAD-CAM blocks and a nanohybrid resin after application of hydrogen peroxide (HP) and resveratrol with 2 extraction media at 24h, 48h, and 72h. Eighteen specimens were obtained from Lava Ultimate (LU), Vita Enamic (VE), and Grandio (GR). Specimens of each material were divided into 3 groups (material only, bleached, resveratrol applied) for 2 extraction media as artificial saliva (AS) and phosphate buffer saline (PBS) (n = 3). For bleached group, 40% HP was applied to specimens for 20min twice. For resveratrol group, 0.5µM resveratrol was applied after bleaching for 10min. Mouse fibroblast cells were exposed to extracts of each group. The viability of cells was determined with MTT assay at 24h, 48h, and 72h. Cell viability data (%) were analyzed statistically using one-way ANOVA, and post hoc Tukey test. Bleached materials showed the lowest cell viability (PBS; p < 0.01/ AS; p < 0.001). There is no statistically significant difference between resveratrol applied and bleached groups (PBS; p = 0.14/ AS; p = 0.072). Regardless of period of time and procedure, GR showed lower viable cell numbers than LU and VE (p < 0.001). Viable cell numbers were higher at 24h than at 72h (p < 0.001). There was no statistically difference between AS and PBS (p > 0.05). For all materials, the application of resveratrol did not affect the cell viability which decreased after bleaching over time. The decrease in nanohybrid resin was more critical than hybrid CAD-CAM blocks. The type of extraction media had no effect on cell viability results.