The Effect of House Dust Mite Immunotherapy, Probiotic and Nigella sativa in The Number of CD4+ IL-4 + Cell, Total IgE level and Asthma Control Test (ACT) Score

Abstract

Ashtma is a chronic inflammatory disorder of the airways dominated by Th2. Immunotherapy was beneficial for ashtma patients. Its long duration was oftenly caused patients to drop out treatment . Probiotic and Nigella sativa as immunomodulator for asthma expectantly could increase the efficacy of immunotherapy . The aims of this study was to evaluate the therapeutic efficacy of immunotherapy combined with probiotics and Nigella sativa in the number of CD4 + IL-4 + cells, Total IgE level and Asthma Scoring Test. A total of 31 children with mild asthma were evaluated and then randomized to receive immunotherapy or immunotherapy plus Nigella sativa or immunotherapy plus probiotic or immunotherapy plus Nigella sativa plus probiotic openly for 14 weeks. Statistical analysis found no significant difference in the mean number of CD4 + IL-4 + cells pre and posttest in all three treatment group. The Total IgE level was decreased significantly in the immunotherapy+ probiotic + Nigella sativa group (p = 0,022). The ACT score were increased in the immunotherapy + Nigella sativa group (p = 0.001), in the immunotherapy + probiotic group (p = 0.004), and immunotherapy + Nigella sativa + probiotic group (p = 0.000). Correlation test found a significant association between the number of CD4 + IL-4 + cells, Total IgE level and ACT score in all groups. The combination immunotherapy, Nigella sativa and probiotics could decrease the Total IgE level thus improve the clinical symptom. Keywords - Immunotherapy, House dust mite, Probiotics, Nigella sativa, CD4 + IL-4 + , Ig E, Asthma Control test