THE EFFECT OF Hg-POLLUTED DRINKING WATER ON THE LEVEL OF HORMONES IN MALE RATS AND THE POTENTIAL THERAPEUTIC ACTION OF THE THIOSEMICARBAZONE AND ANTIPYRINE LIGANDS AND THEIR COMPLEXES

Abstract

Novel Hg(II) complexes of d 10 configuration have been synthesized and characterized by elemental analysis, IR, UV-VIS spectra and thermal analysis. The analytical and spectral data reveal that the ligands (H 2 L 1 - HL 3 ) behave as neutral or mono basic bidentate in nature, coordinating via C=O or C-O and NH or C=N. The harmful effect of Hg-polluted drinking water on male sex hormones, kidney function as well as oxidative status biomarkers of male rats was investigated. Meanwhile, the potential protective effects of synthesized complexes and their ligands were studied. Results showed that orally administration of HgCl 2 for 30 days caused a significant disruption of male sex hormones and kidney function. Further, the level of lipid peroxidation was elevated and activities of antioxidant enzymes were markedly declined in kidney and testes homogenates. The co-administration of HgCl 2 with antipyrine and thiosemicarbazone as well as their complexes for four weeks led to amelioration in the kidney and testes functions as the levels of male sex hormones and kidney function tests were recovered. Meanwhile, these compounds showed ameliorative effects on the oxidative status of rats. It can be concluded that drinking of Hg-polluted water induces oxidative stress pathways that may lead to deterioration in kidney and testes function. The findings also suggest the curative action of antipyrine and thiosemicarbazone as well as their complexes since they exhibited the ability to resist the harmful action of mercury and to protect the organs from the action of free radicals.