Abstract
Summary In the van den Bergh reaction, hemolysis in serum samples results in decreased azobilirubin color development and hence in measured bilirubin levels which are falsely low. The reduction in measured bilirubin content of serum samples ranged from 5 to 15 per cent at discernable levels of hemolysis depending on the volume of serum used in the reaction. No adequate explanation for the effects of hemolysis on the van den Bergh reaction could be found. Qualitative as well as quantitative differences in the effects in individual serum samples make correction of the van den Bergh type of reaction for the presence of hemolysis uncertain at the present time.
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