Abstract
Objective To investigate the effect of halofuginone (HF) on T helper cell 17 (Th17) and relative cytokines following heart transplantation in rats.Methods Three groups were set up:control group,rejection group and HF group.The expression of Th17/interleukin (IL)-17 and the relative cytokines and transcription factor was detected by using real-time quantified polymerase chain reaction (PCR),Western blotting,enzyme linked immunosorbent assay (ELISA) and flow cytometry.The heart grafts were harvested for pathology examination.Results In the control group,rejection group and HF group,IL-17 mRNA expression was 2.18 ±0.23,8.98 ±0.98 and 4.58 ±0.72 at 3rd day; 2.02 ±0.31,6.96 ±0.33 and 4.02 ±0.73 at 5th day,and 2.22 ±0.19,16.96 ±0.33 and 6.22 ±0.58 at 7th day; ROR-γt mRNA expression was 2.62 ± 0.51,8.96 ± 0.33 and 4.02 ± 0.31 at 3rd day,2.31 ± 0.23,5.11 ± 0.47 and 3.87 ±0.32 at 5th day,and 2.85 ±0.43,17.02 ±0.17 and 3.74 ±0.26 at 7th day; interferon (IFN)-γ mRNA expression was 0.92 ±0.05,3.88 ±0.17 and 0.63 ±0.05 at 5th day,and 1.05 ±0.07,6.83 ± 0.24 and 1.21 ±0.25 at 7th day,respectively.In the rejection group and HF group,IL-4 mRNA expression was 0.78 ± 0.04 and 1.67 ± 0.32 at 5th day,and 0.43 ± 0.07 and 3.66 ± 0.21 at 7th day,and transforming growth factor (TGF)-β mRNA expression was 0.38 ±0.07 and 0.58 ±0.03 at 5th day,and 0.21 ±0.02 and 1.31 ±0.17 at 7th day,respectively.There was significant difference at each time point between two groups (P ＜ O.05).The numeber of Th17 cells in the transplanted heart and serum IL-17 level of recipient rats were increased in rejection group,and those in HF group were decreased.HF treatment could prolong the survival time of the transplanted heart (P ＜ 0.05).Conclusion Th17/IL-17 played an important role in the acute rejection after heat transplantation.HF may alleviate the acute rejection through inhibiting the Th17/IL-17 and IFN-γexpression as well as increasing the IL-4 and TGF-[3 expression. Key words: Cardiac transplantation; Allograft rejection; T helper cell 17; Halofuginone
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