Abstract
We aim to explore the influence of Guizhi decoction(GD) on inflammatory response in myocardial ischemia models. 45 New Zealand rabbits were divided into three groups randomly: sham control group, myocardial ischemia group and myocardial ischemia + Guizhi decoction treatment group. The rabbits were treated with Guizhi decoction or saline for 20 days. After that, the myocardial ischemia model was created by left anterior descending coronary artery ligation. Color Doppler Echocardiography computed the ejection fraction (EF). Serum content of MyD88 and TNF-α and concentration of cardiac markers (CK-MB, CK, LDH and cTnT) was measured. After ligation, ST segment was elevated. However, 15 rabbits died due to operation and postoperative care, and were excluded from this study. The final sample size was sham control group (n=10), myocardial ischemia group (n=10) and Guizhi decoction group (n=10) Compared with the sham control group, the ejection fraction was decreased and the expression of the TLR4, MyD88, TNF-α,CK-MB, CK, LDH and cTnT in myocardial ischemia group was increased (p<0.05). After treatment with Guizhi decoction, EF was improved and related inflammatory response factors were decreased (p <0.05). Pretreatment with Guizhi decoction attenuated the level of proinflammatory markers and improved heart function in animal model for myocardial ischemia.
Highlights
Acute myocardial ischemia is the leading cause of death in most nations
We found that compared with the sham control group, the content of the Toll-like receptor 4 (TLR4), MyD88, TNF-α, creatine kinase (CK)-MB, CK, Lactate dehydrogenase (LDH) and cardiac troponin T (cTnT) in myocardial ischemia model group was increased
Studies showed that ischemia and reperfusion after restoration of blood flow resulted in inflammatory response and induced cardiomyocyte apoptosis, which influenced the myocardial remodeling. (Eltzschig & Eckle, 2011) In this study, we found that Guizhi decoction decreased the inflammatory factors, including TLR4, MyD88 and TNF-α in myocardial ischemia rabbits and improved the cardiac function
Summary
Acute myocardial ischemia is the leading cause of death in most nations. Even if the patients survive, the myocardial injury influences the life quality and 5-year survival rate. Ischemia and reperfusion after restoration of blood flow will result in inflammatory response and induce cardiomyocyte apoptosis, which will influence the myocardial remodeling. The myocardial ischemia was recorded in traditional Chinese medicine. They believed that the disease belonged to the “chest pain” or “heartache” category, which mainly showed blood stasis, qi deficiency, phlegm-turbidness, cold coagulation. (Zhou & Wang, 2014) Various traditional Chinese medicines were tested to treat the myocardial ischemia. (Das et al, 2006) Studies showed that some traditional Chinese medicines play important role in cardio-protection through anti- inflammation, such as salvia miltiorrhiza bunge, forsythoside B, schisandra chinensis, ShuMai Decoction and Xiongshao Capsule. Palmatine, extracted from the coptidis rhizome and forsythoside B, extracted from lamiophlomis rotate can improve myocardial function. (Kim et al, 2009; Jiang et al, 2010) Makhana has cardioprotective properties through clearing ROS. (Das et al, 2006) Studies showed that some traditional Chinese medicines play important role in cardio-protection through anti- inflammation, such as salvia miltiorrhiza bunge, forsythoside B, schisandra chinensis, ShuMai Decoction and Xiongshao Capsule. (Jiang et al 2010; Ren et al, 2010; Chiu & Ko, 2004; Yin et al, 2008; Dong-mei et al, 2013)
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