Abstract

AbstractAll enzymes of the citric acid cycle were found to be present in cell‐free extracts of the facultative methylotroph Arthrobacter 2B2 except 2‐oxoglutarate dehydrogenase and pyruvate dehydrogenase. The dehydrogenase (E1) and lipoamide dehydrogenase (E2) component of 2‐oxoglutarate could be detected indicating that the complex was present, but may be very labile. No great differences in enzyme levels were detected between cells grown on trimethylamine and those on multicarbon substrates. Citrate synthase was found to be insensitive to inhibition by NADH or 2‐oxoglutarate, but some inhibition was observed with ATP.

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