Abstract
The effect of indole-3-acetic acid (IAA), gibberellic acid (GA 3) abscisic acid (ABA), kinetin and Ethrel on callose formation in decapitated shoots of Salix viminalis L. was examined using a rapid freezing and specific staining technique. IAA and GA 3, at appropriate concentrations, inhibit the formation of wound callose. The IAA effect can be detected after 30 min and the greatest inhibition was obtained with a concentration of 0.1% IAA in lanolin paste (w/w). Kinetin and ABA had no effect on the system, but Ethrel increased the levels of callose. It is suggested that the formation of definitive callose in phloem as a response to wounding may be mediated by ethylene. The results provide only a partial explanation for the phenomenon of hormone directed transport.
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