Abstract
In this study, we have investigated the effect of copper oxide nanoparticles (CuO-NPs) on callogenesis and regeneration of Oryza sativa L (Super Basmati, Basmati 2000, Basmati 370, and Basmati 385). In this regard, CuO-NPs have been bio-synthesized via Azadirachta indica leaf extract. Scanning electron microscope (SEM) analysis depicts average particle size of 40 ± 5 nm with highly homogenous and spherical morphology. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) have been employed to confirm the phase purity of the synthesized NPs. It is found that CuO-NPs exhibit very promising results against callus induction. It is attributed to the fact that green synthesized CuO-NPs at optimum dosage possess very supportive effects on plant growth parameters. In contrast to callogenesis, differential regeneration pattern has been observed against all of the examined O. sativa L. indigenous verities. Overall observation concludes that CuO, being one of the essential plant nutrients, has greatly tailored the nutritive properties at nano-scale.
Highlights
Plant tissue culture is of prime importance due to its novel and versatile applications in basic and advanced biotechnological research
We have investigated the effect of copper oxide nanoparticles (CuO-NPs) on callogenesis and regeneration of Oryza sativa L (Super Basmati, Basmati 2000, Basmati 370, and Basmati 385)
Callus induction and shoot regeneration are important in cell and tissue culture techniques for plant improvement (Kumar et al, 2009)
Summary
Plant tissue culture is of prime importance due to its novel and versatile applications in basic and advanced biotechnological research. In plant tissue culture aseptic condition is one of the basic requirement. Microbial contamination in tissue cultured plants is one of the major hurdle which leads to the massive loss of platelets. Several reports of silver (Abdi et al, 2008) and titanium oxide (Shiraishi et al, 2009) NPs as antimicrobial agents have been reported. Callus induction and shoot regeneration are important in cell and tissue culture techniques for plant improvement (Kumar et al, 2009). Applications of in vitro transfer techniques to plant improvement depend on regeneration capacity (Higuchi and Maeda, 1990). Callus cultures are significant in plant biotechnology
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