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Abstract
Grazing Alfalfa in the nutrition of ruminant animals has been increasing day by day. In this study, it was aimed to compare milk mineral and fatty acid levels of cows fed with Total Ration Mix (TMR) and cows fed with fresh alfalfa and commercial concentrate feed (2.7 Mcal/kg %21 crude protein). 24 Holstein cows were divided into two groups. The first group (TMR Group) was fed with TMR. TMR content involves barley, commercial concentrate feed (2.7 Mcal/kg %21 crude protein), corn silage, straw, and dry alfalfa. The second group (Aflalfa Group) was mainly fed with artificial alfalfa pasture as well as barley and commercial concentrate feed. The element and fatty acid levels of alfalfa plant samples from artificial alfalfa pasture and milk samples were determined by using Induced Coupled Plasma Mass Spectrophotometry (ICP-MS) and Gas Chromatography (GC) devices. TMR Group's milk calcium (Ca), phosphorus (P), and zinc (Zn) levels were found to be lower than cow's milk in Alfalfa Group. It was observed that while the caprylic acid (8:0) and myristic acid (14:0) levels of the Alfalfa Group were lower than the TMR Group; myristoleic acid (14:1), cis-11-eicosenoic acid (20:1n9c), gamma-linolenic acid (18:3n6), cis-8,11,14-eicosatrienoic acid (20:3n6), cis-5, 8,11,14,17-eicosapentaenoic acid (20:5n3), KLAc9t11 and KLAt10c12 fatty acid levels were high (p <0.05). Alpha-linolenic acid (18:3n3) 14.482%, linoleic acid (18:2n6c) 4.528%, palmitic acid (16:0) 9.551% and stearic acid (18:0) 29.788% were found to be major fatty acids in alfalfa plants. The alfalfa plant was determined at levels of Ca 8167 mg/kg, P 951 mg/kg, manganese (Mn) 14.16 mg/kg, Zn 4.25 mg/kg and iron (Fe) 143 mg/kg. In this study, a high rate of 18:3n3 fatty acids were detected in the alfalfa plant content. For further studies, we think that this study may be a pioneer in the research on the effects of 18:3n3 fatty acid on CLA metabolism in rumen biohydrogenation of cows. As a result, alfalfa is considered to have positive effects on element and fatty acid levels.
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