Abstract
The effect of gramicidin on the phosphorylation rate, electron flow and light-induced H 2+ uptake (ΔH +) in chloroplasts with methyl viologen (MV) or phenazine methosulfate (PMS) added has been studied. In the presence of MV low concentrations of gramicidin (~10 −9 M) were shown to inhibit the phosphorylation rate up to 80–90% of the initial value, without changing either the electron transport rate or ΔH + value. Two components of phosphorylation have been identified in the presence of PMS - the first is DCMU-sensitive, which was suppressed by low gramicidin concentrations (< 10 −9 M) and the second - DCMU-insensitive component, which was suppressed by high gramicidin concentrations (~10 −7 M) exclusively, the high gramicidin concentration inducing the ΔH + value fo fall.
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