The Effect of Gram Negative Bacteria on Semen Parameters of a Sample of Infertile Men and Antibiotic Susceptibility Tests

Abstract

The most Gram statistically significant cause of male infertility is male urogenital tract infection, being related to 8%-35% of male infertility. The detrimental effect of bacterial infection on male infertility was in no whole agreement. The aim of this work showed the influence of Gram negative bacteria on semen quality among infertile males. A total of 50 semen specimens were collected from infertile men and 50 samples from the healthy individual as the control group were attending Kamal Al-Sammrai Hospital / Baghdad during the period 25 May 2020 to 15 October 2020. Bacteriospermia identification in semen specimens was first prepared by the classical culture methods and confirmed by Vitek 2 system. The present study revealed that 38% of isolates were Gram negative and 62% was no growth. Escherichia coli (22%) followed by Enterobacter cloacae (4%), Klebsiella pneumonia (4%), and each of Raoultella ornithinolytica, Pseudomonas aeruginosa, and Citrobacter freundii at a percentage (2%) and only one (1%) unidentified.  This work indicated that some Gram negative bacteria lead to poor health of seminal fluid. Record of Gram negative bacilli was sensitive to Vancomycin, Rifampicin, and Trimmethoprim/sulfamethoxazole combination drug while Fusidicacid was resistant from most Gram negative bacilli. Semen investigation for bacterial detection in infertile males should be done regularly because bacteria may be hurting the semen quality.