Abstract
This investigation has shown the following hierarchy of chloroplast reactions which were susceptible to increasing glutaraldehyde concentration given for 6 min at 0° C. (1) Cyclic and non-cyclic photophosphorylation was completely inhibited by 100 mM glutaraldehyde. (2) Reduction in the rate of H+ uptake, and increase in the rate of H+ efflux occurred to give a ratio uptake/loss of unity with 100 mM glutaraldehyde. (3) Volume fixation occurred at 200 mM glutaraldehyde. (4) Fixed chloroplasts were resistant to stimulation of H+ and electron transport by weak acid anions and uncouplers. (5) Light-induced electron-transport activity progressively decreased. About 35% inhibition of the basal (non-phosphorylating) rate and 60% inhibition of the uncoupled rate occurred on fixation. (6) The extent of the light-induced H+ changes were reduced only by about 10% upon fixation.
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