The effect of glutamine on Dehydroepiandrosterone-induced polycystic ovary syndrome rats

Gengxiang Wu,Jing Yang,Xue Hu,Jinli Ding

doi:10.1186/s13048-020-00650-7

Abstract

BackgroundPrevious studies have shown that chronic inflammation and oxidative stress may play an important role in the pathophysiology of polycystic ovary syndrome (PCOS), and glutamine (Gln) have showed the anti-inflammatory and antioxidant properties. So the aim of this study is to investigate the effect of glutamine supplementation on PCOS rats.MethodsFemale Sprague–Dawley rats were randomly assigned into four groups (n = 10 /group), control group, PCOS group, PCOS+ 0.5 g/kg Gln group and PCOS+ 1.0 g/kg Gln group. All the PCOS rats were administrated with 6 mg/100 g dehydroepiandrosterone (DHEA) for 20 consecutive days, all the PCOS+Gln groups were intraperitoneal injected glutamine twice in the next morning after the last DHEA injection. All the samples were collected 12 h after the last administration. Ovarian histological examinations were analyzed and the concentration of serum hormone, inflammatory and oxidative stress factors were measured.ResultsThere was no obvious ovarian histological change among the PCOS group and PCOS+Gln groups. All the detected inflammation factors [C-reactive protein, interleukin (IL)-6, IL-18, tumor necrosis factor] showed significantly higher in all the PCOS groups compared to the control group (P < 0.01), and were significantly decreased with the supplementation of 0.5 g/kg glutamine (P < 0.01). Concentrations of superoxide dismutase were significantly lower in all the PCOS groups (P < 0.01) compared to the control group, and increased significantly with the supplementation of 0.5 g/kg glutamine (P < 0.01). Serum concentrations of malondialdehyde, nitric oxide synthase and nitric oxide were significantly higher in PCOS group (P < 0.01) compared with the control group, and significantly decreased to the comparative levels of control group with supplementation of 0.5 g/kg glutamine (P < 0.01).ConclusionThere is low-grade inflammation and oxidative stress in DHEA-induced PCOS rats. The supplementation of 0.5 g/kg glutamine could effectively ameliorate the inflammation and oxidative stress conditions of PCOS.

Highlights

Polycystic ovary syndrome (PCOS) is one of the most common endocrine diseases in women of reproductive age, with a prevalence ranging from 9 to 18% [1]
The ovaries in polycystic ovary syndrome (PCOS) group and PCOS+Gln groups showed a large number of cystic dilatation and fewer corpus luteums, there was no obvious change among the PCOS group and PCOS+Gln groups (Fig. 1)
Serum hormones Serum concentrations of T and luteinizing hormone (LH) were significantly higher in all the PCOS groups than the control group (Fig. 2a, c), and the concentrations of T were increasing with the dosage of supplementation of glutamine, but the concentrations of LH were decreasing with the dosage of supplementation of glutamine

Summary

Introduction

Polycystic ovary syndrome (PCOS) is one of the most common endocrine diseases in women of reproductive age, with a prevalence ranging from 9 to 18% [1]. This syndrome is characterized by ovulatory dysfunction, polycystic ovarian morphology, insulin resistance and hyperandrogenism [2]. Previous studies have shown that chronic inflammation and oxidative stress may play an important role on the pathophysiology of PCOS [2,3,4]. Previous studies have shown that chronic inflammation and oxidative stress may play an important role in the pathophysiology of polycystic ovary syndrome (PCOS), and glutamine (Gln) have showed the antiinflammatory and antioxidant properties. The aim of this study is to investigate the effect of glutamine supplementation on PCOS rats

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Discussion

Conclusion