Abstract
Rabbit kidneys were perfused with a high K+ and Mg++ solution as previously described (J S R 21(3);169,1976). Briefly the animals were anesthetized with urethane and pentobarbital and the left kidney perfused at 37ºC with a previously dialyzed solution in an artificial heart‐lung machine custom fabricated. Several functional physiological parameters were evaluated such as perfusate and urine flow, GFR, %TNa, %TK, osmolar clearance, perfusion pressure and vascular resistance. The experiments lasted 2 hours. To measure kidney function creatinine was added to the perfusate and Na,K, and osmolarities were measured every ten minutes. Urinary flow increased 6.5‐fold in AG group when compared to control. Glutamine increased this parameter 16.5 times. The same trend was observed with GFR that was enhanced 5.5 times in the AG group while Glut 1 increased 10.6%. The fractional sodium reabsorption increased 8.87% with AG while Glut increased 11.26%. No changes were observed in the fractional transport of potassium. We conclude that these 2 substrates promote a great improvement in kidney functional preservation in this ex vivo model. These substrates could be important metabolic support when perfusing kidney for transplantation.Financial support: CNPq and FUNCAP Brazil
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