Abstract
Sucrose hydrolyses rates are independently regulated from glucose phosphorylation and uptake rates. Whereas sucrose hydrolysis increases with substrate concentration, glucose uptake rates remain relative constant. The resultant free glucose accumulation obtained from high sucrose hydrolyses rates and relatively constant and much lower glucose uptake rates antagonize the stimulatory effect of sucrose on levan sucrase. Using repeated fed-batch cultivation systems, it could be demonstrated that glucose inhibits sucrose hydrolysis and ethanol inhibits glucose uptake.
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