Abstract
Glucose metabolism disorders contribute to the development of various diseases. Numerous studies show that these disorders not only change the normal values of biochemical parameters but also affect the mechanical properties of blood. To show the influence of glucose and poloxamer 188 (P188) on the mechanical properties of a red-blood-cell (RBC) suspension, we studied the aggregation of the cells. To show the mechanisms of the mechanical properties of blood, we studied the effects of glucose and poloxamer 188 (P188) on red-blood-cell aggregation. We used a model in which cells were suspended in a dextran 70 solution at a concentration of 2 g/dL with glucose and P188 at concentrations of 0–3 g/dL and 0–3 mg/mL, respectively. RBC aggregation was determined using an aggregometer, and measurements were performed every 4 min for 1 h. Such a procedure enabled the incubation of RBCs in solution. The aggregation index determined from the obtained syllectograms was used as a measure of aggregation. Both the presence of glucose and that of P188 increased the aggregation index with the incubation time until saturation was reached. The time needed for the saturation of the aggregation index increased with increasing glucose and P188 concentrations. As the concentrations of these components increased, the joint effect of glucose and P188 increased the weakening of RBC aggregation. The mechanisms of the observed changes in RBC aggregation in glucose and P188 solutions are discussed.
Highlights
Research shows that incubation of Red blood cells (RBCs) in glucose solutions causes oxidation of membrane lipids and glycation of proteins, which leads to a reduction of RBC aggregation [9,12]
Oxidation and glycation affect changes in RBC aggregation; the results presented in this paper suggest that glucose transport to erythrocytes plays a key role in the temporal changes in the aggregation of these cells
The results presented in this study show that, for RBCs placed in glucose solutions, the aggregation index increases with the time of incubation in these solutions
Summary
The metabolic diseases plaguing today’s civilization result from damage to normal metabolisms, especially in cases such as diabetes mellitus, in which the carbohydrate pathway is disrupted by inappropriate insulin secretion or abnormal responses by the body. With this disruption of the carbohydrate pathway, there is an elevated level of glucose in the blood, called hyperglycemia. Glucose has an effect on RBCs, changing their biochemical properties and the ability of these cells to aggregate. It is important to understand the simultaneous effects of glucose and drug polymers on RBC aggregation
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