Abstract
The use of deltamethrin and cypermethrin insecticides to control pests has been implemented in Indonesia. Irregular and excessive use of insecticides can have toxic effects on the male reproduction system. Deltamethrin and cypermethrin insecticides can inhibit androgen biosynthesis and disrupt the growth of sperm. Therefore, this research is conducted to find out the effect of deltamethrin and cypermethrin insecticides toward the level of intratesticular testosterone in male Wistar strain rats. This research is conducted in vivo using male Wistar strain rats. Nine rats are divided into three groups of treatment, namely control group, deltamethrin group with 0.26 mg/kg of weight, and cypermethrin group with 0.26 mg/kg of weight. Treatment is conducted every day for 15 days orally, and then the rats are dissected to take their testicles. Testicles are chopped and their intratesticular homogenates are taken by adding the medium of DMEM and collagenase (0.25 mg/mL). The measurement of the level of testosterone is conducted by using Electrochemiluminescence immunoassay (ECLIA) method. Data on the change of the increase of body weight, organ weight, and the level of testosterone is analyzed statistically using the one-way ANOVA test. The research result shows that giving 0.26 mg/kg of weight of deltamethrin and 0.26 mg/kg of weight of cypermethrin does not affect the increase in body weight, relative weight of the reproductive organ, and the level of intratesticular testosterone of male Wistar strain rats.
Highlights
IntroductionThe occurrence of insect pests is often a very detrimental problem (Boadu et al, 2011)
In agriculture, the occurrence of insect pests is often a very detrimental problem (Boadu et al, 2011)
A pyrethroid is included in synthetic analog pyrethrin insecticide, a natural compound found in the dried extract of white chrysanthemum flowers (Chrysanthemum cinerariae folium)
Summary
The occurrence of insect pests is often a very detrimental problem (Boadu et al, 2011). A pyrethroid is included in synthetic analog pyrethrin insecticide, a natural compound found in the dried extract of white chrysanthemum flowers (Chrysanthemum cinerariae folium). Pyrethroid insecticide, including deltamethrin and cypermethrin, has antiandrogenic effects in vitro (Du et al, 2010; Fang et al, 2013). Other research reports that deltamethrin and cypermethrin insecticides have effects that disrupt spermatogenesis (Fang et al, 2013; Orlu, 2014). The spermatogenesis process in the body needs testosterone hormone, while pyrethroid can cause antiandrogenic effects by interfering with the biosynthesis and secretion of androgens in the body, including testosterone (Fang et al, 2013). This research aims to find out the comparison between giving deltamethrin and cypermethrin insecticides (at a dose which farmers use to control pest) toward the production of intratesticular testosterone of male Wistar strain rats
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