The Effect of Gibberellin on Somatic Embryo Growth and Maturation and Plantlet Regeneration of Tangerine (Citrus reticulata Blanco.) var. Batu 55

Elok Rifqi Firdiana,Wahyu Widoretno,Serafinah Indriyani

doi:10.21776/ub.jels.2015.005.01.04

Elok Rifqi Firdiana, Wahyu Widoretno + Show 1 more

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https://doi.org/10.21776/ub.jels.2015.005.01.04

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Abstract

The effect of gibberellin at multiplication stage on somatic embryo growth and maturation and plantlet regeneration of tangerine ( Citrus reticulata Blanco.) var. Batu 55 was assessed. Somatic embryo at globular phase was cultured on MT media + 30 gL -1 sucrose and various concentrations of gibberellin (0, 2, 4, 6, and 8 ppm). The somatic embryo was maturated on MT media + 500 ppm malt extract + 73 mM sorbitol + 73 mM galactose. Cotyledonary somatic embryo was regenerated into plantlet on MT media + 500 ppm malt extract + 30 gL -1 sucrose + 2 ppm GA 3 . The results showed that the addition of gibberellin in somatic embryo multiplication stage increased somatic embryo growth and maturation and plantlet regeneration of tangerine. Optimum concentration of gibberellin needed for somatic embryo growth was 4 ppm which yielded two-fold fresh weight compared to control. The percentage of maturation was very low below 5%. The addition of gibberellin in media at embryo multiplication stage slightly increased the percentage of maturation about 1-2%. Thirty percent of cotyledonary embryo was able to regenerate into plantlet. The addition of gibberellin in media at embryo multiplication stage increased the regeneration percentage, even the addition of 8 ppm gibberelline yielded regeneration percentage up to 70%. Keywords: gibberellin, growth, maturation, plantlet regeneration, somatic embryo

Highlights

Gibberellin treatment Somatic embryo at globular phase was cultured on MT media + 30 gL-1 sucrose + various gibberellin concentrations (0, 2, 4, 6, and 8 ppm)
The highest fresh weight was reached by the addition of 4 ppm gibberellin in culture media, almost twice as control
The percentage of maturation was very low below 5%

Summary

INTRODUCTION

Batu 55 is one of local citrus varieties that has several special characters such as sweet, easy to peel, and almost seedless. These characters cause the high demand of tangerine, but it is still unfulfilled by national production. The plants regenerated from somatic embryo had long juvenile period [1]. Along with this reason, the endeavor to multiply seeds with shorter juvenile period was important. It was important to assess the effect of gibberellin on somatic embryo growth and maturation and plantlet regeneration of Tangerine var.

MATERIALS AND METHODS

RESULT

Findings

CONCLUSION