The effect of gamma irradiation on the quality and natural antioxidants of fenugreek and lupine seeds in hyperlipidemic rats

Abstract

The present study aims to evaluate the effect of gamma irradiation on thequality and natural antioxidants of fenugreek (Trigonellajoenum-greacum) and lupine (lupinus terms) seeds at dose levels of 10 and 20 kGy in hyperlipidemic rats. Rats were divided into: group (1) fed on balanced diet (negative control), group (2) fed on high fat high cholesterol (HFHC) diet (positive control), groups (3,4,5,6,7 and 8) fedon HFHC diet with either 15% non-irradiated or irradiated at dose levels of 10 or 20 kGy fenugreek or lupine seeds, respectively for eight weeks. The resultsshowed that the applied doses of non-irradiated or irradiated fenugreek or lupine seeds at dose levels of 10 or 20 kGywere significant decrease in relative liver weight except for group (3), PER except for group (4), food intake, final body weight, gain in body weight, serum alanine amino transferase (ALT), serum aspartate amino transferase (AST), total cholesterol, triacylglycerol, low density lipoprotein-cholesterol (LDL-C), malodialdehyde and nitric oxide. On the other hand, it was observed that there were significant increase in FER except for group (5), blood hemoglobin, high density lipoprotein-cholesterol (HDL-C), superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT)when compared with HFHC diet (group 2).