The Effect Of Fresh Human Amniotic Membrane Storage Time On Growth Factor Levels (EGF, TGF-B, bFGF) in Freeze-Dried Human Amniotic Membranes

Abstract

Background: Each preservation method can cause different levels of damage to tissue components and can affect the regenerative performance of the amniotic membrane. The concentrations of most of the cytokines were found to decrease during the preservation period. There are still no studies regarding the effect of fresh human amnion storage time on growth factor levels in freeze dried human amnion products. Method: This study used an experimental post test group design. Result: There was no significant difference in EGF levels of freeze-dried human amniotic membrane products resulting from 3 types of fresh human amniotic membrane storage (1 week storage with 8.49 ± 5.16 pg/mL, 3 months with 4.55 ± 1.98 pg/mL, and 9 months with levels of 4.04 ± 2.12 pg/mL). There was no significant difference in bFGF values between freeze-dried human amniotic membrane products resulting from 3 types of storage duration of fresh human amniotic membrane (1 week storage with levels of 97.86 ± 113.98 pg/mL, 3 months with levels of 109.69 ± 113.39 pg/mL, and 9 months with levels of 117.53 ± 73.46 pg/mL). There was a significant difference in the levels of TGF-b, namely in the product from storage for 1 week (97.93 ± 18.27 pg/mL) and 3 months (55.94 ± 28.67 pg/mL). Whereas TGF-b levels in freeze dried human amniotic membrane from the results of fresh human amniotic membrane storage at 1 week and 9 months, and 3 months and 9 months were found to have no significant difference. Conclusion: There was no significant difference in EGF and bFGF levels in this study. There was a significant difference in the levels of TGF-b freeze-dried human amniotic membrane produced from fresh human amniotic membrane with higher growth factor levels at 1 week of storage compared to 3 months.