Abstract
1. 1. The effect of folate deficiency on the incorporation of radioactivity from 14C-labelled serine into protein and other oviduct constituents of oestrogen-treated immature chicks was studied. 2. 2. In two experiments oviduct slices were incubated with L-[U- 14C] serine and subsequently post-incubated with L-[3- 3H] serine in the first experiment and with unlabelled serine in the second. The radioactivity of specific tissue fractions was determined. 3. 3. Folate deficiency resulted in a reduced rate of incorporation of radioactivity into the protein fraction of the oestrogen-stimulated oviduct. 4. 4. In the second experiment, on post-incubation in a non-radioactive medium, the cold TCA-soluble fraction rapidly decreased in radioactivity, while for the hot TCA-soluble fraction radioactivity did not appear to decline.
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