Abstract
The purpose of the study is to explore the effect of flue-curing procedure on the diversity of microbial communities in tobaccos and the dynamic change of compositions of microbial communities in the flue-curing process. It expects to provide a theoretical basis for the application of microbes in tobacco leaves and a theoretical basis and idea for optimization of the flue-curing technologies. By investigating tobacco variety K326, the tests were carried out for comparing the conventional flue-curing procedure and dry-ball temperature set and wet-ball temperature degradation flue-curing procedure. Based on the culture-independent approach and high-throughput sequencing procedure, the relationship between the flue-curing procedure for tobaccos and microbial communities in tobaccos was revealed by measuring the dynamic change of microbial communities. The results indicated that:(1) Relative to surface wiping method, washing method was more suitable for the sampling of microbes on the surface of tobacco leaves; (2) Dry-ball temperature set and wet-ball temperature degradation flue-curing procedure was more favorable for maintaining the microbial diversity of tobaccos; (3) Relative to bacteria of the tobaccos, the succession rule of the fungal communities in tobaccos was relatively steady; (4)Compared with bacterial community diversity, the fungal community diversity presented an obvious negative correlation with temperature and humidity during the flue-curing process. (5) The function of bacterial communities in tobaccos matched with the material transformation law of tobaccos, having a direct correlation on the flue-curing process. In short, Dry-ball temperature set and wet-ball temperature degradation flue-curing procedure can more favorably maintain the microbial diversity of tobaccos; moreover, the function of the tobacco system involved in microbes in tobaccos was closely related to the material transformation law of tobaccos in the flue-curing process. It validated that the bacteria in tobaccos play an important role in the flue-curing process of tobaccos.
Highlights
The purpose of the study is to explore the effect of flue-curing procedure on the diversity of microbial communities in tobaccos and the dynamic change of compositions of microbial communities in the flue-curing process
Dry-ball temperature set and wet-ball temperature degradation flue-curing procedure can more favorably maintain the microbial diversity of tobaccos; the function of the tobacco system involved in microbes in tobaccos was closely related to the material transformation law of tobaccos in the flue-curing process
By analyzing the distribution of operational taxonomic units (OTUs) of surface and endophytic bacteria of tobacco leaves, it can be seen that the abundance of the surface bacteria was remarkably higher than that of endophytic bacteria in the flue-curing process; this indicated that the endophytic bacteria of tobaccos were more sensitive to temperature and humidity and the reduction rate of OTUs of endophytic bacteria was obviously larger than that of surface bacteria in the flue-curing process
Summary
The purpose of the study is to explore the effect of flue-curing procedure on the diversity of microbial communities in tobaccos and the dynamic change of compositions of microbial communities in the flue-curing process. Based on culture-independent method and high-throughput sequencing procedure, the effect of the flue-curing procedure on, and the dynamic change of, the microbial diversity of tobaccos were revealed by measuring the dynamic change of microbial communities; the mechanism of increasing the quality of tobacco leaves by microbes in the flue-curing stage was analyzed. It provides a theoretical basis for the application of microbes in tobacco leaves and a new evaluation index for the maturity of flue-cured tobacco leaves, expecting to further improve the flue-curing procedure for tobaccos
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