The effect of fermented Glycine max (L.) Merr. to rats induced CCl4 toward hepatic IL-6 expression and ALT-AST level

Abstract

Carbon tetrachloride (CCI4) is one of the hepatotoxic compounds that can cause hepatic fibrosis. Fermented soybean (Glycine max (L.) Merr.) has higher isoflavone and fibrinolytic enzymes that could degrade fibrin substrates. The aim of this research was to know the effect of fermented soybean (Glycine max (L.) Merr.,) to IL-6 expression and ALT-AST level in rats (Rattus novergicus) that induced by CCl4. The groups consist of negative control group, positive control group, 3 treatment groups which used dose 200, 400, 800 mg/kg BW. The treatments were conducted 2 mL/day along 14 days. Rats were euthanized then liver and serum were collected. The IL-6 expression of liver was measured by Immunohistochemistry and ASTALT level used spectrophotometer. The parameters were analyzed statistically quantitatively using one-way ANOVA and continued test of Post hoc Tukey (α = 0,05). The result showed that dose of 800 mg/kg BW was an effective dose which has the highest percentage reduction both of hepatic IL-6 expression (68,10%) and ALT-AST level 81,16% and 46,45% respectively. The effect was related isoflavone from fermented soybean that acted as anti-inflammatory. The conclusion was the fermented of soybean able to decrease the hepatic IL-6 expression and ALTAST level in induced CCl4 rats.