The effect of feeding antioxidant rich coffee on glucose blood response, MDA and SOD on diabetic rats induced with streptozotocin

Abstract

This study aimed to examine the effects of antioxidant rich coffee on blood pressure, glucose blood response, levels of MDA and SOD serum, and liver tissue of Wistar diabetic rats. The study used 24 Wistar rats in which 18 rats were induced with Streptozotocin of 60 mg/kg BW for 4 days to make them diabetic (fasting blood glucose level >250 mg/dL). Rats than divided into 4 groups: 1) control group (non-diabetic group), given distilled water, 2) diabetic control group, given sugar cane coffee of 0.45 gram/200 g BW/day, 3) diabetic group, given antioxidant rich coffee of 0.45 g/200 g BW/day, 4) diabetic group, given antioxidant rich coffee of 0.90 gram/200g BW/day. Weekly observations were made on blood pressure, fasting blood sugar levels, body weight and blood pressure, as well as serum and tissue levels of SOD and MDA. The results showed that coffee rich in antioxidant significantly reduced oxidative stress in diabetic rats with lower blood pressure, serum MDA values, and the tissue MDA value than that in the diabetic rats given sugar cane coffee. This illustrates that consuming 0.90 g/day antioxidant rich coffee in mice, or the equivalent of 60 g/day in humans, can reduce oxidative stress in diabetes.