The effect of exercise on the contractile properties of single skinned fast- and slow-twitch skeletal muscle fibres from the adult rat.

Abstract

The effects of long-term endurance exercise on the contractile properties of single skinned muscle fibres from adult rats, were investigated. Adult (4-month-old) male rats were subjected to a 16-week, high-intensity endurance swimming programme, where animals carried a load (corresponding to 2% of body wt), during all 2-h training sessions. At the conclusion of the training period, muscle fibres isolated from the extensor digitorum longus (EDL), and soleus (SOL), could be classified into distinct classes or fibre types on the basis of their Ca(2+)- and Sr(2+)-activated contractile characteristics. The fast-twitch EDL comprised two fibre populations, while the slow-twitch SOL was found to be composed of three distinct fibre types. Endurance swimming modified the contractile characteristics of fibres from both the EDL and SOL, but exerted greater influence on those of the SOL. This was illustrated by significant increases in the sensitivity to Ca2+ and Sr2+, and a lower threshold for contraction by these activating ions, in the exercised group. Not one of the total of 272 fibres sampled, exhibited mixed fast- and slow-twitch contractile characteristics, often associated with exercise-induced fibre type transformations. Thus, high-intensity endurance swimming induced changes in some single muscle fibre contractile properties of adult rats, but did not cause major changes in fibre type distribution.