THE EFFECT OF EXCRETORY/SECRETORY PRODUCTS FROM DIBOTHRIOCEPHALUS DENDRITICUS PLEROCERCOIDS OF GULL TAPEWORM AND LIGULA INTERRUPTA LIGULA ON INFLAMMATORY ACTIVATION OF MACROPHAGES

Abstract

In this paper, the anti-inflammatory potential of excretory/secretory products (ESP) from plerocercoids of D. dendriticus gull tapeworm and L. interrupta ligula was estimated for the first time in the model of LPS-induced activation of macrophages in vitro. A monocyte cell line derived from a patient with acute monocytic leukemia, THP-1, was used as a macrophage model. Cells were differentiated into macrophages using phorbol myristate acetate. The anti-inflammatory properties of ESP were determined by measurement of pro-inflammatory Th1-cytokines TNF and IL-6, as well as anti-inflammatory Th2-cytokines IL-4 and IL-10, in the incubation medium using commercial kits for Enzyme-Linked Immunosorbent Assay. The results of our study demonstrated that overnight incubation of macrophages with ESP from plerocercoids of L. interrupta at a concentration of 100 μg/ml led to a twofold decrease in the content of Th1-cytokines TNF and IL-6 in medium during LPSinduced activation of macrophages. Pre-treatment of macrophages with ESP from plerocercoids of D. dendriticus overnight did not affect the content of Th1-cytokines TNF and IL-6. The results of our study indicated that ESP from L. interrupta has a pronounced anti-inflammatory effect, while ESP from D. dendriticus does not have such an effect. At the same time, the mechanism of the anti-inflammatory action of ESP from L. interrupta has not been elucidated and requires further research.