The effect of ethanolic extract of mangosteen peel to malondialdehyde level, colony number, and pulmonary damage level score of tuberculosis model rats

Abstract

Mycobacterium tuberculosis is an intracellular pathogen that can replicate in host macrophages. This research examined the medication mechanism of mangosteen peel extract as anti-tuberculosis towards rats infected by Mycobacterium tuberculosis H37Rv. The mangosteen peel has chemical antituberculosis properties, such as xanthone, flavonoid, and tanin. Rats were invected by M. tuberculosis 105 (100 μl) by intratracheal for 4 weeks, then divided into 6 experimental groups: K1 (infected rats were treated by standard feed); K2 (infected rats were treated by INH); P1 (infected rats were treated by INH and extract 100mg/kg BW/day); P2 (infected rats were treated by INH and extract 200mg/kg BW/day); P3 (infected rats were treated by INH and extract 300mg/kg BW/day); and P4 (infected rats were treated by INH and extract 400mg/kg BW/day). INH and extract were administered orally for 4 weeks. On the 29th rats were euthanized to collect the left lung for MDA measurement and germs planting, and the right lung was collected for Hematoxyline eosin preparation. The data was analyzed by SPSS. The mangosteen peel ethanolic extract showed significant differences between treatments towards Tuberculotic rats treated by INH. The MDA level decreased with a value of p=0,000 (p<0,005) r= 82,2% ; the decrease of colony count with a value of p=0,000(p<0,005) r= 93,3%; the decrease of pulmonary damage level score with p=0,001(p<0,005) r= 50%. Mangosteen peel ethanolic extract reduced MDA levels, colony counts, and lung damage levels at a dose of 200mg/kg BW/day of tuberculosis-model rats.