The effect of esterase enzyme on aging dental composites

Abstract

We measured the push-out and diametral tensile strength of dental restorative composites following aging under environmental conditions relevant to the oral cavity; air (A), artificial saliva (AS), acidified (50 mM CH3 COOH, pH = 4.7) artificial saliva (AS + HAc), and AS with esterase enzyme (AS + ENZ). Cylindrical test specimens (6.3 mm diameter by 5.1 mm long) were prepared by placing 0.3 g of nanofilled composite in an epoxy ring and cured. Twenty samples were aged in each environment for 163-186 days at 37°C. The push-out strengths (mean ± standard error of the mean [SEM], in MPa) for specimens were: A-2.4 ± 0.2, AS-7.3 ± 0.5, AS + HAc-7.2 ± 0.9, and AS + ENZ-6.0 ± 0.6. Following the push-out test, the diametral tensile strength and elasticity were immediately determined. The diametral tensile strengths (mean ± SEM, in MPa) for specimens were: A-54.0 ± 1.6, AS-31.4 ± 1.3, AS + HAc-34.3 ± 1.2, and AS + ENZ-22.5 ± 0.7. The push-out strength was lowest for the A environment due to shrinkage of the composite. The push-out strength increased significantly as water diffused into the specimens (AS and AS + HAc) but decreased significantly in the enzyme environment (AS + ENZ). The diametral tensile strength was highest for specimens in the A environment, which was significantly higher than both the AS and AS + HAc specimens and > 2× higher than the AS + ENZ specimens. The results indicated that a water environment (with or without acid) caused a significant decrease in the mechanical properties of this composite, but the greatest decrease was seen in water with esterase. This is the first study to demonstrate that esterase enzymes affect the bulk strength of a commonly used commercial dental composite. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2178-2184, 2019.