The effect of Escherichia Coli induction on superoxide dismutase (SOD) and Malondialdehyde (MDA) levels in acute rhinosinusitis white rats models

Abstract

Background: Acute Rhinosinusitis is an inflammation of the paranasal sinuses and the nasal cavity lasting no longer than 6 weeks. One of the etiologies is the gram-negative bacteria, the lipopolysaccharide layer containing bacteria Escherichia coli. Lipopolysaccharide was the leading cause of inflammatory mechanisms, and subsequent Reactive Oxygen Species production was thought of as the agent that injure the cells thorough the oxidative stress. This study aimed to determine the effect of E-coli induction on the ratio of SOD and MDA levels in acute rhinosinusitis white rats model.Methods: This study used an experimental post-test only control group design with white rats strain that met the inclusion and exclusion criteria divided into two groups. The study includes 8 rats as control groups and 32 rats in the treatment group. Treatment includes induction of rhinosinusitis by E-coli. SOD and MDA levels measured from retro-orbital venous blood samples were measured as marker oxidative stress on 7th, 14th,21st, and 28th days. Data were analyzed using SPSS version 22 for Windows.Results: There were significant differences in SOD and MDA levels from day 7 to day 28 between the control and the intervention groups (p<0.001). The mean SOD levels on days 7, 14, 21, and 28, were lower than those of the control group. The MDA levels from day 7 to day 28 of the intervention group was significantly higher than that of the control group (p<0.001). Spearman Correlation test obtained a significant correlation between SOD and MDA levels (r = -0.604; p<0.001 on day 7 and r = -0.453; p< 0.003)Conclusion: There is a significant correlation between the effect of induced E coli on SOD and MDA levels in acute rhinosinusitis white rats models.