Abstract
Mesenchymal Stem Cells (MSCs) are one of the most important elements of bone marrow microenvironment, which has a role in stromal support and stem cell differentiation. Exosomes are small vesicles that responsible from various cellular roles such as cell-cell communication and cell signaling, which may affect nearby and distant cells/tissues. MSCs have therapeutic importance because of their multipotency and immune modulation potentials also with their exosomes. Erythropoietin (Epo), produced by liver hepatocytes, is responsible for erythroid differentiation (erythropoiesis) in bone marrow. In addition, Epo treatment of several cell types including MSCs, showed therapeutic effects in various diseases. Objective:The aim of this study is to examine the effect of erythropoietin on bone marrow MSCs transcriptome and exosome derived miRNA profile. Materials and Methods:Effect of 3 different doses of Epo (1 IU/ml, 10 IU/ml and 100 IU/ml) for 48 hours on MSCs transcriptome profile was analyzed. The results illustrated that 10 IU/ml Epo treatment has the most effective concentration in terms of gene expression profile. Therefore, small RNA libraries targeting miRNA was analyzed with 10 IU/ml Epo treated versus non treated groups with next generation sequencing. Results:We found that Epo treatment slightly changed global gene expression profile. On the other hand, it was observed that Epo treated MSCs have different exosomal miRNA profile. Conclusion:Differentially expressed exosomal miRNAs may have therapeutic effects in different conditions. It will be important to perform further studies with in vitro models, mimicking different physiological conditions and diseases for MSCs and exosome biology.
Highlights
Mesenchymal Stem Cells are one of the most important elements of bone marrow microenvironment, which has a role in stromal support and stem cell differentiation
It was noted that 4 miRNAs were present in the control group, they were not in exosomes as a result of Epo treatment (Table 4)
It is important to clarify the content of these exosomes, as it is shown in previous studies that Mesenchymal Stem Cells (MSCs)-induced exosomes may have a therapeutic potential [3,4,5]
Summary
Mesenchymal Stem Cells are one of the most important elements of bone marrow microenvironment, which has a role in stromal support and stem cell differentiation. Objective: The aim of this study is to examine the effect of erythropoietin on bone marrow Mesenchymal Stem Cells transcriptome and exosome derived miRNA profile. A glycoprotein hormone, allows the differentiation of hematopoietic stem cells in the bone marrow into erythroid precursor cells (erythropoiesis) [14]. This function is stimulated through the receptor called EpoR. It was observed that different cell types such as neuron, endothelium, and cardiomyocytes were started to express EpoR as a result of treatment with Epo [12] This suggests that Epo is effective in addition to erythropoiesis. It has been shown in studies that Epo has an effect on neural, muscle and cardiovascular tissues
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
