THE EFFECT OF ERBISOL ON PRODUCTION OF IL-1β AND NITRIC OXIDE (NO) BY CELLS OF IMMUNE SYSTEM IN PATIENTS WITH CHRONIC UROGENITAL CHLAMYDIOSIS IN VITRO

Abstract

Infection caused by Chlamydia trachomatis is on the special place among sexually transmitted diseases, due to high incidence of chronic urogenital chlamydiosis (CUGC), social importance of the problem and difficulties in treatment. In spite of an active use of new medicaments, the number of CUGC patients continues to grow therefore the search for new measures of immunocorrection is also in progress. Aim: to determine in vitro the functional activity of cells of monocyte-and-macrophage series in CUGC patients by the production of IL-1β and nitric oxide under the action of immunocorrecting medicament Erbisol. Materials and methods: the cytokine production was determined through studying the spontaneous and induced synthesis of IL-1β by cells of CUGC patients in vitro by means of immunoenzymatic method employing the test system DIACLONE (France) and immunoenzymatic analyzer STAT-Fax Plus-303 (USA). The content of stable metabolite NO-ionic nitrite-NO2 - in supernatants of cells of monocyte and macrophage series was determined by means of spectrophotometry method using the standart Greiss-reagent. Results: the IL-1β production by cells of healthy donors was 44.9±1.29 pg/ml, while the meanindex of spontaneous IL-1b production in CUGC patients was statistically higher than in healthies – 156.5±6.2 pg/ml. The activation of immunocompetent cells by mitogen in patients led to statistical reducing of monokin production and averaged 119.0±5.0 pg/ml (p 0.05). The spontaneous NO secretion in patients exceeded 2.5 times the NO level in healthy donors, the induction by Erbisol led to higher elevation of nitric oxide level in patients – about 3 times as compared with spontaneous production in healthies and 2 times exceeded the NO production induced by mitogen. Conclusion: the lack of an increase in IL-1β production during activation by mitogens in CUGC patients in vitro, in contrast to healthies, can be considered as decrease in the reserve potential of cells of monocyte-macrophage series against the background of their high activation. Erbisol demonstrates the potential increase in IL-1β and NO production by patient’s cells, that can be considered as the positive effect for the perspective empoying of the medicament in a situation when during a long time the immune system cannot ensure the effective resistance and destroy pathogen-Chlamydia trachomatis.