Abstract

Endometriosis is a gynecological disease that endometrial cells develop outside the uterus. This event happens when the endometrial glands grow outside the endometrium and uterine muscles, especially in the pelvis. Although endometriosis is widespread, the clinical manifestations of the disease are very different, and it is challenging to adapt to the conventional classification system to divide patients into homogeneous groups. Given the importance of endometriosis, a correct, accurate, and timely diagnosis of this disease can significantly prevent its complications. Using health-related software is one of these ways. Enhanced Endometriosis Archiving Software (ENEAS) is a web-based application based on one of the most widely used open-source database management systems (MySQL), allowing the direct link to other open-source software for data management and storage. In the current study, the effect of ENEAS application was considered in patients with endometriosis, and its influence on IL-8 and MCP-1 gene expression was evaluated. For this purpose, 100 women with endometriosis were divided into two groups of 50 patients. The first group (control group) was examined by a gynecologist and received medication and treatment. In the case group, their demographic and clinical information were entered into ENEAS software. To study the expression of the IL-8 gene and MCP-1 gene, after collecting 5 ml of blood samples in tubes containing anticoagulant, RNA extraction was performed by Total RNA Purification Kit (Cat. 17200, 37500, 17250). Then cDNA synthesis was performed for this purpose, and a Bioneer DNA synthesis kit (South Korea) was used. The results showed that the expression level of the IL-8 gene in the case group was significantly reduced compared to the control group (P = 0.035). MCP-1 gene expression was also decreased compared to the control group, but this decrease was not significant. Therefore, those who used this application for treatment had reduced expression of IL-8 and MCP-1 genes. This event indicates that this application has reduced the amount of inflammation caused by endometriosis with proper analysis.

Highlights

  • Developing a global registry of endometriosis bio-banks and databases is one of the main priorities for endometriosis research. For this purpose, Enhanced Endometriosis Archiving Software (ENEAS) has been developed as the first databank to store, retrieve, compare, and correlate data collected on the management of patients diagnosed or suspected endometriosis in different centers [14]

  • Based on the importance of health-related software [14], this study investigated the effect of ENEAS application on the expression of Interleukin 8 (IL-8) and MCP-1 genes in patients with endometriosis

  • Developing a global registry of endometriosis biobanks and databases is one of the main priorities for endometriosis research

Read more

Summary

Introduction

The results showed that the expression level of the IL-8 gene in the case group was significantly reduced compared to the control group (P = 0.035). MCP-1 gene expression was decreased compared to the control group, but this decrease was not significant. Those who used this application for treatment had reduced expression of IL-8 and MCP-1 genes. Endometrial critical mediator in angiogenesis and has strong tissue deposition is most common in the pelvis potential in physiological and pathological This disorder is one of the most wellspace, rectovaginal septum) [2]. Endometriosis is a known conditions for changes in angiogenesis [5]

Methods
Results
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call