The effect of encomir-93 mimic transfection on the expression of miR-93 and PSA and androgen receptor in prostate cancer LNcap cell line.

Abstract

Prostate cancer (PCa) is one of the most common cancers in men with high mortality rate which is a major concern for men's health. However, the molecular mechanisms remain poorly understood. miR-93 is an important oncogene which may have important function inprostate cancer.So, this study aimed to predict that encomir-93 mimic transfection on the expression of miR-93 and PSA and AR in prostate cancer LNcap cell line. Lymph node carcinoma of the prostate (LNCaP) was cultured and then miR-93 mimics wasdesigned, synthesized and the transfected to LNCaP. Theexpression level of prostate-specific antigen (PSA) andandrogen receptor (AR) was determined via Real-time PCR after treated with 15pmol of miR-93 mimics. miR-93 mimic transfection led to significant increase in PSA and AR expression in comparison with control group (p≤0.05). The miR-93 and its target genes has important role in PCa progression via enhancement in PSA and AR expression. Further research on the function of the miR-93 and its target genes in tumorgenesis and progression PCa could be helpful for the treatment of prostate cancer.