Abstract
The article considers the scientific aspects of probable partial transformation of lactose into lactobionic acid due to the electrical discharge dispersion of magnesium and manganese conductive granules in milk whey – a traditional lactose-containing raw material. The object of this study was milk whey, defatted and with casein dust particles removed, which was treated in a discharge chamber with a conductive layer of magnesium and/or manganese granules at (20±2)°С with 120–180 s of exposure. A UPLC-MS/MS analysis of milk whey was carried out using a TSQ Vantage chromatograph-mass spectrometer (ThermoFinnigan, USA) connected to a Dionex Ultimate 3000 liquid chromatograph. The changes have been studied taking place in pH and redox potential of milk whey after electrical discharge treatment, as compared to the original whey. It has been established that electrical discharge treatment with 180 s of exposure increases the Mg content in milk whey by approximately 4 times, and increases the Mn content by 3.5 times. The dispersed metal particles are found in nano- (about 30 nm) and microscale (100 nm to 10 µm). The UPLC-MS/MS analysis of the test samples of whey that underwent electrical discharge treatment allowed identifying a chromatographic peak [M–H]–=357 m/z related to lactobionic acid. The derivative lactose content increased by 2 times in the whey samples treated for 180 s in a reaction chamber with a conductive layer of Mg between the corresponding electrodes, as compared to the original whey, and by 4 times in the samples subsequently treated in the reaction chambers with a layer of Mg and Mn granules between the corresponding electrodes, with 120 s of exposure in each chamber. The way has been presented of solving the problem of complex, economically practical and environmentally safe processing of milk whey with the prospect of obtaining lactobionic acid, a biologically valuable derivative of lactose.
Highlights
Formulation of the problemStudying the directed impact on one of the main components of whey in order to obtain its derivatives is an important world-class problem [1,2], which is given constant attention by the International Dairy Federation
The average particle size in the colloidal solution was 118±5 nm for magnesium and 270±11 nm for manganese. These results indicate the preconditions for a specific-purpose transformation of lactose into lactobionic acid in milk whey that underwent electrical discharge treatment
Basing on the changes in physical and chemical parameters, the preconditions have been determined for the specific-purpose transformation of lactose into lactobionic acid by electrical discharge dispersion of magnesium and manganese conductive granules in whey
Summary
Studying the directed impact on one of the main components of whey (lactose) in order to obtain its derivatives is an important world-class problem [1,2], which is given constant attention by the International Dairy Federation. Due to the unique physical, chemical, and biotechnological properties of lactose derivatives, galactose, glucose-galactose syrups, lactulose, lactitol, lactobionic acid, etc., they are widely used in the production of functional foods and in medicine [1,3]. It is a relatively new product that is obtained by lactose oxidation. It has significant potential as a promising biologically active compound. LBA is a promising functional and technological ingredient for the production of food. The commercial significance of lactobionic acid, which has recently grown rapidly in the world, has led to the development of new effective ways of obtaining and using it
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