Abstract
The viability and performance of Saccharomyces cerevisiae which had been subjected to high voltage electric fields is assessed. Suspensions of mixed strains of S. cerevisiae were electrostatically sprayed through a charged nozzle into an inert solvent, collected and used for the fermentation of malt extract liquor. Biomass, substrate conversion and ethanol production kinetics were determined as a function of nozzle voltage. Yeast performance was unaffected by exposure to high tension fields. Within the limits of experimental error, cell viability and ethanol production rates for the cell batches exposed to high voltage and for the control batches were the same. The results suggest the possibility of using electrostatic spraying to augment mass transfer and phase separation processes in two phase biotransformation systems.
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