The Effect of either Aerobic Exercise Training or Chrysin Supplementation on Mitochondrial Biogenesis in Skeletal Muscle of High Fat Diet-Induced Obese Mice

Abstract

PURPOSE The purpose of this study was to investigate the effects of either aerobic exercise or resveratrol supplementation on mitochondrial biogenesis in skeletal muscle of the high fat diet-induced obese mice. METHODS C57BL/6 (4 weeks aged) male mice were divided into four groups; normal diet group (NC, n=10), high fat diet group (HC), high fat diet group with chrysin supplementation (HCh, n=10) and high fat diet with exercise group (HE, n=10). Aerobic exercise was performed on a treadmill exercise for 40-60 min/day at 10-14 m/min, 0% grade, 4 days/week for 16 weeks. Chrysin (50 mg/kg body weight) daily administrated orally at 4 days/week frequency for 16 weeks. RESULTS The gene expression of SIRT-3 in HE group was significantly increased compared to that in HC group (p<.05). Also, PGC-1α and COX-IV mRNA levels in the HE group were the highest among all groups (p<.05). CONCLUSIONS It seems that high fat diet-induced obese dose not affect mitochondrial biogenesis gene expression in skeletal muscle. However aerobic exercise training could increase the mitochondrial biogenesis gene expression of skeletal muscle in high fat dietinduced obese mice. These findings suggest that only aerobic exercise not chrysin supplementation has a positive effect on mitochondrial biogenesis in the skeletal muscle in high fat diet-induced obese mice. Keywords: Aerobic exercise, Chrysin, Mitochondrial biogenesis, Obese, Skeletal muscle