The effect of egg phosphatidylcholine on antibiotics retention and release from hydroxyapatite

Abstract

Hydroxyapatite (HAP) has been used in orthopedics as an aid in bone grafts following surgery of bone tumors and osteoarthrosis, often promoting osteoconduction. The incorporation of antibiotics into HAP beads to prevent local infection would seem to be therapeutically effective. This report describes in vitro and in vivo release rate of cefotiam hydrochloride (CTM, 3.5 mg/bead) loaded into HAP beads (8.48 mm in diameter). Egg phosphatidylcholine (EPC) was also incorporated into HAP to control the rate of release of CTM. In vitro, 100% of CTM was released within 3 h from the EPC-free HAP beads. The rate of diffusion of CTM from HAP was prlonged with the incorporation of EPC. In vivo, 100% of CTM of the EPC-free CTM was released within 7 h but the rate of release of CTM from the EPC-CTM was extended. In vivo, the serum level of CTM reflected the rate of release of the antibiotics from the HAP bead. We conclude that EPC is useful in controlling the diffusion rate of CTM from HAP. © 1992 John Wiley & Sons, Inc.