The effect of e-cigarettes smoking on expression and methylation of CYP1A1 and CYP1B1 genes and other biochemical parameters

Abstract

Electronic cigarettes (e-cigs) are becoming increasingly common. Sixty samples of superficial scrape were collected to evaluated expression and Methylation of CYP1A1 and CYP1B1 genes, and ninety we collected biological fluid serum, and assessed various biomarkers in study groups including (30 with e-cig users (Vaping), 30 with tobacco cigarette (smoker), and 30 of control group), age range of 18 – 35 years old. The study was conducted between November 2020 and March 2021 samples were collected from Cafe and outdoor restaurants. The results of this study revealed that ROS (ng/ml), and MDA (µmol/I) were significantly higher e-cigs (vaping), and smoker groups than in control group, while Vitamin D3(ng/ml), and Vitamin E (μg/mL) were significantly lower in e-cigs (vaping), and smoker groups than those of control group, whilst the results showed that the vaping group has the least level of CYB4A1 gene expression (0.05) then followed by the group that smoking normal cigarette (0.37), when compared them to the control group, while expression here with CYB4B1 showed insignificant difference between vape users (mean = 0.289) and the smokers of the normal cigarette (mean = 0.40) when compared to the control group which has expression value equal to 1, Finally, the methylation of the CYP4A1 gene shown lower percentage of methylation by the vaping group (5%) and then followed by the smokers (17.7%) and the higher methylation shown in the non-smokers (24.45%).