The Effect of Dried Amnion Membrane Application in the Expression of Platelet Derived Growth Factors in the Healing Process of Stomach Stab Wound (Study on New Zealand Rabbit)

Abstract

Background: Management of penetrating abdominal trauma, especially those affecting the stomach, keepsdeveloping. Although the technology in the field of surgery is getting more advanced, the risk of complications dueto leakage is still common. A lot of research is being done to improve the tissue healing process, including a varietyof surgical techniques and material uses. The amniotic membrane is a material that is widely used to help stimulatethe healing process. The amniotic membrane contains growth factors, one of which is platelet-derived growth factor(PDGF). PDGF is a major player in the wound healing process.Purpose: To investigate the differences in PDGF levels in gastric rupture repair with dry amniotic membrane asa biological dressing compared to primary repair of gastric rupture without using dry amniotic membrane.Method: This research was an experimental analytical study, using 42 samples of rabbits which were groupedinto 2 groups, namely the control and treatment groups. Stab wound of 2 cm by 0.5 cm wide with a depth entire gastricwall in gastric corpus was done and repaired with 4-6 interrupted suture using 5/0 polypropylene monofilament. Inthe control group, the wound was only sutured and the treatment group, dry amniotic membrane was applicatedbefore suturing. The expression of PDGF was examined from the suture tissue on day-7.Result: Intensity score was obtained in the treatment group with the highest score of 2 with a sample of 11(52.4%) as well as in the control group as many as 13 (61.9%) samples who had a score of 2. The extensionscore was obtained in the treatment group with the highest score of 1 with a total sample size of 10 (47.6%)while the control group had the highest score with a score of 0.5 as much as 14 (66.7%). After obtaining thePDGF intensity and extension values, then these two values are multiplied to get the PDGF expression. Fromthe results of the comparison test using the Mann Whitney test, it was found that the difference in PDGFexpression was significant between the treatment group and the control group with p = 0.008 (p <0.05).Conclusion: Applying dry amniotic membrane to the wound on the stomach will increase the expression ofPDGF in the wound area.