Abstract
The aim of disinfection is to reduce the number of microorganisms on surfaces which is a challenge due to biofilms. In the present study, six quinolone resistant Escherichia coli (QREC) strains with three different biofilm matrix compositions were included to assess the log10 colony forming units (CFU) reduction effect of three disinfectants at various exposure times on biofilm of different ages and morphotypes. Biofilm was formed on stainless steel coupons for two and five days before transferred to tubes with Virocid 0, 25%, VirkonS 1%, and TP990 1% and left for various exposure times. The biofilms were scraped off and serial dilutions were spread on blood agar plates where colony forming units (CFU) were counted. A mean log10 CFU reduction ≥4 was seen on two-day-old biofilm with VirkonS and Virocid (30 min) but not on five-day old biofilm. TP990 did not display sufficient effect under the conditions tested. The bactericidal effect was inferior to that reported on planktonic bacteria. The findings of this study should be considered when establishing both disinfectant routines and standard susceptibility tests, which further should accommodate E. coli biofilms and not only Pseudomonas as is the case today.
Highlights
World health organization (WHO) considers antibiotic resistance to be one of the biggest threats to global health, food security, and development today, and it focuses on reducing resistance to antimicrobials defined as critically important for human medicine, e.g., quinolones and fluoroquinolones [1,2]
We found that the effect of the disinfectants was influenced both by their composition and the exposure time, as well as by the age of the biofilms and the constituents in the biofilm matrices
Our results show that increasing exposure times is beneficial when combatting quinolone resistant Escherichia coli (QREC) in biofilm, at least concerning certain disinfectants
Summary
World health organization (WHO) considers antibiotic resistance to be one of the biggest threats to global health, food security, and development today, and it focuses on reducing resistance to antimicrobials defined as critically important for human medicine, e.g., quinolones and fluoroquinolones [1,2]. Bacterial resistance to these compounds has increased yearly from 2013 to 2016 and is continuing to rise in Europe. Failure to eradicate resistant bacteria from the food production environment may contribute to persistence and dissemination
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