Abstract
Abstract The presence of diphenylamine (DPA) during growth of mutant strains of Phycomyces blakesleeanus caused the expected inhibition of the formation of unsaturated carotenes, and the accumulation of phytoene in all cases. Cell extracts from DPA-grown cultures incubated with [2- 14 C]mevalonic acid, also exhibited these effects. Inhibition of in vitro carotenogenesis was similarly shown by extracts from normally-grown mycelia, incubated with DPA. Removal of DPA from mycelia or from cell extracts, resulted in the formation of unsaturated carotenes. The ratio of 15- cis -all- trans -phytoenes from mycelia grown ± DPA was only marginally altered, but, in vitro , the presence of DPA caused a significant increase in the formation of the all- trans isomer in the C5 strain. These results indicate that DPA acts by post-translational regulation of enzymic activities.
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