Abstract
The NADPH-dependent oxidation of ethanol by rat liver microsome preparations was studied in the presence of azide to inhibit the peroxidatic activity of catalase. Dimethylsulfoxide, benzoate, mannitol and thiourea, four compounds that react rapidly with hydroxyl radicals, each inhibited the oxidation rate of ethanol. Inhibition was competitive with respect to ethanol. In contrast, urea, a compound that reacts poorly with hydroxyl radicals, was essentially without effect. Dimethylsulfoxide at concentrations that inhibited the oxidation of ethanol had no effect on the xanthine oxidase-mediated oxidation of ethanol or on aniline hydroxylase or aminopyrine demethylase activity of microsomes. These results suggest that ethanol oxidation by microsomes can be dissociated from drug metabolism and that the mechanism of ethanol oxidation may involve, in part, the interaction of ethanol with hydroxyl radicals that are generated by microsomes during the oxidation of NADPH.
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