Abstract
Two DCM mutations (E40K and E54K) of Tropomyosin (Tm) were examined using the thin-filament extraction and reconstitution technique. Effects of Ca2+, ATP, phosphate and ADP concentrations on force and its transients were studied at 25°C and compared to WT. Both E40K and E54K showed significantly lower high-Ca (pCa 4.66) tension (THC, 1.21±0.06 and 1.24±0.07, respectively), low-Ca (pCa 7.0) tension (TLC, 0.07±0.02 and 0.06±0.02 at pCa 7.0), and Ca2+ activatable tension (Tact=THC-TLC, 1.15±0.08 and 1.18±0.06) compared to WT (THC=1.53±0.07, TLC=0.12±0.01, Tact=1.40±0.07) (±sem, N=8-21 for all studies).
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